In veterinary artificial insemination (AI), liquid nitrogen (LN) plays a crucial role in the cryopreservation and storage of semen. Proper storage ensures sperm viability for long periods, making successful AI possible even weeks, months, or years after semen collection.
Liquid nitrogen is an extremely cold, inert liquid (-196C / -321F) used to freeze and preserve semen in cryogenic containers (dewars). It halts all biological activity in sperm cells, maintaining their structure and function until thawing and insemination.
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